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| HILIC-HPLC-ELSD测定山药中尿囊素的含量 |
| Determination of Allantoin in Rhizoma Dioscoreae by HILIC-HPLC-ELSD |
| 投稿时间：2014-08-12 修订日期：2015-01-15 |
| DOI： |
| 中文关键词: 亲水作用色谱 蒸发光散射检测 山药 尿囊素 |
| 英文关键词:HILIC ELSD Rhizoma Dioscoreae Allantoin |
| 基金项目: |
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| 中文摘要: |
| 摘 要 目的： 建立山药中尿囊素的HILIC-HPLC-ELSD含量测定方法。方法: 色谱柱为Waters XBridge HILIC (150 mm×4.6 mm,5 μm)；流动相为乙腈-水（85∶15）；流速为0.8 ml·min-1；雾化压力：40 psi；漂移管温度：80℃；雾化器温度：50℃。结果:尿囊素在1.5～7.5 μg范围内线性关系良好(r=0.998 1)，线性方程为Y=1.67X+2.35，检出限为25 ng, 平均回收率为99.9%，RSD=0.72%(n=9)。 结论: 该方法简单、可靠，可用于山药中尿囊素的质量控制。 |
| 英文摘要: |
| ABSTRACT Objective: To establish an HILIC-HPLC-ELSD method for the determination of allantoin in Rhizoma Dioscoreae. Methods: The separation was performed on a Waters XBridge HILIC column (150 mm×4.6 mm, 5 μ m), the mobile phase was acetonitrile water (85∶15) with the flow rate of 0.8 ml·min-1, and the nebulizer pressure was 40psi, the drifting tube temperature was 80℃ and the nebulizer temperature was 50℃. Results: The linearity of allantoin was good within the range of 1.5-7.5 μg(r=0.998 1). The regress equation was Y=1.67X+2.35,the detection limit was 25 ng, and the average recovery of allantoin in Rhizoma Dioscoreae was 99.9% with RSD of 0.72%(n=9). Conclusion:The method is simple and reliable, which can be used in the determination of allantoin in Rhizoma Dioscoreae. |
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