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| HPLC法测定复方苯妥英钠凝胶剂中克林霉素磷酸酯的含量 |
| Determination of Clindamycin Phosphate in Compound Sodium Phenytoin Gels by HPLC |
| 投稿时间：2014-08-16 修订日期：2014-11-11 |
| DOI： |
| 中文关键词: 克林霉素磷酸酯 复方苯妥英钠凝胶剂 高效液相色谱法 |
| 英文关键词:Clindamycin phosphate Compound phenytoin sodium gels HPLC |
| 基金项目:2011年湖北省教育厅重点科研项目（编号：D20112006） |
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| 中文摘要: |
| 摘 要 目的： 建立HPLC法测定复方苯妥英钠凝胶剂中克林霉素磷酸酯的含量。方法: 采用ZORBAX SB C18色谱柱（250 mm×46 mm，5 μm）；流动相为0.1 mol·L-1的磷酸二氢钾（用磷酸调pH 2.5) 乙腈(75∶25)；流速0.8 ml·min-1；检测波长210 nm；柱温：25℃；进样量10 μl。结果:克林霉素磷酸酯在3.00～18.00 μg范围内线性关系良好(r=0.999 5)；平均回收率为101.11%(RSD=0.34%, n=6)。结论: 本方法简单，灵敏，重复性好，可用于复方苯妥英钠凝胶剂中克林霉素磷酸酯的含量测定。 |
| 英文摘要: |
| ABSTRACT Objective: To establish an HPLC method for the determination of clindamycin phosphate in compound phenytoin sodium ge1s. Methods: The HPLC analysis was carried out on a ZORBAX SB C18 column （250×46 mm, 5 μm）with 0.1 mol·L-1 KH2PO4 solution (adjusting pH to 2.5 with H3PO4 solution) acetonitrile (75∶25) as the mobile phase at the flow rate of 0.8 ml·min-1．The detection wavelength was 210 nm, the column temperature was 25℃ and the injection volume was 10 μl. Results: The linear range of clindamycin phosphate was 3.00-18.00 μg（r=0.999 5）．The average recovery was 101.11%(RSD=0.34%, n=6). Conclusion:The method is simple，sensitive and reproducible, and can be used in the determination of clindamycin phosphate in compound phenytoin sodium gels. |
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