| ABSTRACTVarious new ophthalmic formulations have been prepared to enhance ocular bioavailability, while lots of irritant substances are applied, including surfactants, polymers and other cationic materials. Therefore, safety of the preparations has been paid more and more attention besides the efficacy. Since in vitro experiments lack of associated enzymes and integral reaction of organization, the reasonability and correlation of the results extrapolated to the animal experiments still need to be further proved. The in vivo common eye irritation methods (winking, histopathological slices, Draize score and confocal microscopy test etc) were reviewed, evaluated and analyzed in the paper. |
