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| 虎杖配方颗粒的HPLC指纹图谱研究 |
| Study on HPLC Fingerprint of Polygonum Cuspidatum Dispensing Granules |
| 投稿时间：2014-10-28 修订日期：2014-12-12 |
| DOI： |
| 中文关键词: 虎杖 配方颗粒 高效液相色谱法 指纹图谱 虎杖苷 大黄素 |
| 英文关键词:Polygonum cuspidatum Dispensing granules HPLC Fingerprint Polydatin Emodin |
| 基金项目: |
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| 中文摘要: |
| 摘 要 目的： 建立虎杖配方颗粒的HPLC指纹图谱，为虎杖配方颗粒的质量控制和评价提供依据。方法: 采用高效液相色谱 线性梯度洗脱法，测定了12批不同厂家的虎杖配方颗粒的HPLC色谱图，并建立了指纹图谱；色谱条件为 SunFire C18色谱柱 （250 mm×4.6 mm，5 μm），流动相为乙腈（A）和0.05%磷酸溶液（B），流速：1.0 ml·min-1，柱温: 30 ℃，检测波长：230 nm，进样量：10 μl。结果:采用国家食品药品监督管理总局推荐的“中药色谱指纹图谱相似度评价系统(2004A版)”计算处理，通过HPLC指纹图谱分析，虎杖配方颗粒有14个共有的色谱峰，相似度均大于0.98，并确认2个已知峰为虎杖苷和大黄素。结论: 本研究所建立的虎杖配方颗粒HPLC指纹图谱稳定性、重复性好，可作为虎杖配方颗粒质量质量控制和评价提供参考。 |
| 英文摘要: |
| ABSTRACT Objective: To establish the HPLC fingerprint for Polygonum cuspidatum dispensing granules. Methods: The HPLC fingerprint of 12 batches of Polygonum cuspidatum from different manufacturers were determined. The analysis was performed on a Waters SunFire C18 column（250 mm×4.6 mm，5 μm) with acetonitrile as the mobile phase A and 0.05％ phosphoric acid solution as the mobile phase B with gradient elution. The flow rate was 1.0 ml·min-1, the detection wavelength was 230 nm, the column temperature was 30℃，and the injection volume was 10 μl.Results: The results were calculated according to “similarity evaluation system for traditional Chinese medicine chromatographic fingerprint” nominated by CFDA combined with the analysis of the HPLC fingerprints. Totally 14 common peaks with similarity above 0.98 were found in the HPLC fingerprint of Polygonum cuspidatum, including the peak respectively for polydatin and emodin. Conclusion:The method can provide more information for the quality control of Polygonum cuspidatum dispensing granules. |
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