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| 华东覆盆子根中没食子酸的提取工艺优选及含量测定 |
| Extraction Technology Optimization and Content Determination of Gallic Acid in the Roots of Rubus Chingii Hu from Eastern China |
| 投稿时间：2014-10-28 修订日期：2014-12-31 |
| DOI： |
| 中文关键词: 华东覆盆子根 没食子酸 提取工艺 含量测定 高效液相色谱法 |
| 英文关键词:Roots of Rubus chingii Hu from eastern China Gallic acid Extraction technology Assay HPLC |
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| 中文摘要: |
| 摘 要 目的： 优选华东覆盆子根中没食子酸的提取工艺，并建立其含量测定方法。方法: 采用高效液相色谱法测定华东覆盆子根中没食子酸的的含量。通过正交设计试验考察料液比、提取时间和盐酸浓度对没食子酸得率的影响。结果:没食子酸在4.082～40.815 μg·mL-1(r=0.999 9)范围内呈良好的线性关系；平均加样回收率为101.28%，RSD=1.08%（n＝6）。最佳提取工艺为料液比1∶〖KG-*2〗15，加热回流2.5 h，盐酸浓度4.2%。结论: 优选出的提取工艺简单可行，建立的含量测定方法稳定可靠，可用于提取和测定华东覆盆子根中的没食子酸。 |
| 英文摘要: |
| ABSTRACT Objective: To optimize the extraction technology of gallic acid in the roots of Rubus chingii Hu from eastern China and establish an assay method. Methods: The content of gallic acid was determined by HPLC. Orthogonal design was used to investigate the influence of solid/liquid ratio, extraction time and concentration of hydrochloric acid on the yield of gallic acid．Results: A good linearity of gallic acid was within the range of 4.082-40.815 μg·mL-1(r=0.999 9), and the average recovery was 101.28%(RSD=1.08%,n＝6）. The optimal technology was as follows: extracted with heating circumfluence in 4.2% hydrochloric acid solution with solid/liquid ratio of 1∶15 for 2.5h. Conclusion:The optimal extraction technology is simple and feasible，and the assay method is stable and reliable，which can be used in the extraction and determination of gallic acid in the roots of Rubus chingii Hu from eastern China. |
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