| ABSTRACT Objective: To observe the protective effect of Shenkang injections in the rats with kidney injury induced by gentamicin. Methods: Forty eight male SD rats were randomly divided into the control group,model group and treatment group with 16 ones in each.The rats were given gentamicin with intraperitoneal injection (100 mg·kg-1·d-1) for 7 days in the model group and the treatment group, and the rats in the treatment group were given Shenkang injections with intraperitoneal injection additionally (2.0 g·kg-1·d-1).Eight rats were sacrificed respectively on the 8th and 15th day in each group,and the detection of serum level of creatinine and cystatin C,urinary concentration of kidney injury molecule-1 (Kim-1) and neutrophil gelatinase associated lipocalin (NGAL),activity of nuclear factor-κβ and Caspase-3,and expression of Bax mRNA and Bcl-2 mRNA in renal tissues as well as the observation of renal tissue apoptosis in each group were conducted.Results: Compared with those in the control group,the serum level of creatinine and cystatin C,urinary concentration of Kim-1 and NGAL,renal tissue apoptosis,activity of nuclear factor-κβ and Caspase-3,expression of Bax mRNA and Bcl-2 mRNA in renal tissues on both 8th and 15th day were higher in the model group (P<0.01).The serum level of creatinine and cystatin C,urinary concentration of Kim-1 and NGAL, renal tissue apoptosis,activity of s nuclear factor-κβ and Caspase-3, expression of Bax mRNA in renal tissues in the treatment group were lower at the two time points than those in the model group, while the expression of Bcl-2 mRNA in homogenate renal tissues were higher in the treatment group than that in the model group at the two time points (P<0.01). All indices were higher at two time points in the treatment than those in the control group. Conclusion: Shenkang injections exhibit protective effect in rats with kidney injury induced by gentamicin through inhibiting renal tissue apoptosis. |
