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| HPLC法测定治疣胶囊中丹参酮ⅡA和丹酚酸B的含量 |
| Determination of TanshinoneⅡA and Salvianolic Acid B in Zhiyou Capsules by HPLC |
| 投稿时间：2014-09-11 |
| DOI： |
| 中文关键词: 治疣胶囊 丹酚酸B 丹参酮Ⅱ A |
| 英文关键词:Zhiyou capsules Salvianolic acid B TanshinoneⅡA |
| 基金项目:湖北省卫生厅中医药科研项目（编号：2010Z B05） |
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| 中文摘要: |
| 摘 要 目的： 建立测定治疣胶囊中丹参酮ⅡA和丹酚酸B的HPLC方法。方法: 采用Waters Atlantis@T3色谱柱(150 mm×4.6 mm，3 μm)，以乙腈-醋酸铵水溶液（用甲酸调至pH=2.4）为流动相梯度洗脱，流速为1.0 ml·min-1，检测波长为260 nm。结果:丹酚酸B、丹参酮ⅡA分别在1.20～60.00 μg·mL-1、0.40～20.00 μg·mL-1之间呈良好线性关系，r=0.999 7、0.999 8。两者的平均回收率分别为101.53%（RSD=1.14%）和95.17%（RSD=4.33%）。结论: 本方法高效、便捷、准确，可用于治疣胶囊的质量控制。 |
| 英文摘要: |
| ABSTRACT Objective: To establish a method for the determination of tanshinoneⅡA and salvianolic acid B in Zhiyou capsules by HPLC. Methods: The chromatographic column was Waters Atlantis@T3 (150 mm ×4.6 mm, 3 μm) using acetonitrile and aqueous ammonium acetate solution (adjusting pH to 2.4 with formic acid) as the mobile phase with gradient elution, the flow rate was 1.0 ml·min-1 and the detection wavelength was 260 nm. Results: The contents of salvianolic acid B and tanshinoneⅡA showed good linear relationship within the range of 1.20-60.00 μg·mL-1(r=0.999 7) and 0.40 20.00 μg·mL-1(r=0.999 8)， respectively. The average recovery was 101.53%（RSD=1.14%）and 95.17%（RSD=4.33%）, respectively. Conclusion:The method is efficient, convenient and accurate, which can be used in the quality control of Zhiyou capsules. |
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