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| 畲药嘎狗粘质量标准的建立 |
| Quality Standard for Traditional She Medicine Herba Desmodii |
| 投稿时间：2014-09-16 修订日期：2014-12-03 |
| DOI： |
| 中文关键词: 畲药；嘎狗粘；质量标准；槲皮素 山柰素 |
| 英文关键词:Traditional She medicine Herba Desmodii Quality standard Quercetin Kaempferol |
| 基金项目:丽水市重点科技创新团队项目（编号：2012CXTD09-05，2012CXTD11-03） |
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| 中文摘要: |
| 摘 要 目的： 研究嘎狗粘的质量标准，为制定合理的质控方法提供科学依据。方法: 运用显微特征、薄层色谱法、高效液相色谱法对嘎狗粘中各成分进行定性、定量测定；对醇溶性浸出物、水分和总灰分进行测定。结果:嘎狗粘显微特征、薄层色谱鉴别特征明显；槲皮素、山柰素分别在0.002～0.050 μg（r＝1.000 0）、0.004～0.100 μg（r＝0.999 9）范围内呈线性，平均加样回收率分别为97.89%（RSD＝0.89%，n=6），98.93%（RSD＝1.31%，n=6）。结论: 研究所得方法简便、准确、重复性好，可有效地控制嘎狗粘的质量。 |
| 英文摘要: |
| ABSTRACT Objective: To study the quality standard for Herba Desmodii, and provide the scientific basis for a reasonable method of quality control. Methods: With the methods of microscopic identification, thin layer chromatography and high performance liquid chromatography for the qualitative and quantitative determination of Herba Desmodii,the ethanol soluble extracts, moisture content and total ashes of Herba Desmodii were determined. Results: The characteristics of thin layer chromatography were obvious; a good linearity of quercetin and kaempferol was within the range of 0.002-0.050 μg（r＝1.000 0） and 0.004-0.100 μg（r＝0.999 9） with the average recovery of 97.89%（RSD＝0.89%，n=6） and 98.93%（RSD＝1.31%，n=6）, respectively. Conclusion:The method is simple, accurate and reproducible, which can effectively control the quality of Herba Desmodii. |
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