| ABSTRACT Objective: To establish an UHPLC-MS/MS method to determine the contents of baicalin, scutellarin, wogonin, caffeic acid, ephedrine, belamcandin, irisflorentin and baicalein in Shegan mixtures. Methods: The chromatographic separation was performed on a ZORBAX SB C18 column （2.1 mm×50 mm，1.8 μm）with the mobile phase consisting of acetonitrile and water (0.1% formic acid), the flow rate was 0.3 ml·min-1, and the column temperature was 35℃. Electrospray ionization source (ESI) was used with multiple reaction monitoring (MRM) combined with positive and negative scanning switch. Negative ion mode detection was used for caffeic acid, belamcandin and scutellarin , while positive ion mode detection was used for baicalin, wogonin, irisflorentin, baicalein and ephedrine.Results: The quantification limit of baicalin, scutellarin, wogonin, caffeic acid, ephedrine, belamcandin, irisflorentin and baicalein was 1.44×10-4, 4.20×10-3, 2.95×10-4, 7.80, 4.90×10-3, 4.6×10-2, 3.18×10-4 ng·mL-1 and 4.85 ng·mL-1. The detection limit was 4.32×10-5, 1.3×10-3, 8.84×10-5, 0.77, 2.90×10-4, 3.33×10-4, 9.5×10-5 ng·mL-1 and 1.46 ng·mL-1, respectively. The correlation coefficients (R2) were all higher than 0.992 3 within the linear ranges. Both intra and inter day RSD were less than 5%. The average recoveries of the eight components were within the range of 80% 120%. Conclusion:The method is simple, rapid, sensitive and accurate. It can be used to determine the contents of baicalin, scutellarin, wogonin, caffeic acid, ephedrine, belamcandin, irisflorentin and baicalein in Shegan mixtures for the quality control. |
