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| 双指标对蒺藜药材质量控制的方法研究 |
| Double index Method for Quality Control of Tribulus Terrestris L. |
| 投稿时间：2014-11-06 修订日期：2014-12-30 |
| DOI： |
| 中文关键词: 蒺藜 蒺藜皂苷元 总皂苷 含量测定 高效液相色谱法 比色法 |
| 英文关键词:Tribulus terrestris L. Saponin Total saponins Content determination HPLC Colorimetry |
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| 中文摘要: |
| 摘 要 目的： 采用HPLC法结合比色法对蒺藜药材质量进行控制。方法: HPLC法采用色谱柱Welch Ultimate LP-C18(250 mm×4.6 mm，5 μm)；流动相：甲醇-水(80∶20)；检测波长：203 nm，比色法采用高氯酸法，以蒺藜皂苷元为指标，对蒺藜皂苷元、总皂苷进行含测方法研究。结果:HPLC法和比色法中，蒺藜皂苷元在0.820～7.380 μg和24.600～86.100 μg有良好的线性关系，平均回收率分别为99.3%、99.5%（n=6）。并对18个产地的蒺藜样品中蒺藜皂苷元和总皂苷含量测定。结论: 所建立的方法灵敏、准确、重复性好，可作为蒺藜药材的质量控制方法。 |
| 英文摘要: |
| ABSTRACT Objective: To establish the quality control method for Tribulus terrestris L. by colorimetry and HPLC.Methods: The HPLC method was with a Welch Ultimate LP-C18 column (250 mm×4.6 mm, 5 μm), the mobile phase was methanol water (80∶20) and the detection wavelength was 203 nm. The colorimetry was with a perchloric acid method. The saponins of Tribulus terrestrist as the index, the determination method for total saponins and saponins of Tribulus terrestris L. was established. Results: The results of the HPLC and colorimetry methods showed saponins of Tribulus terrestris had good linear relationship within the range of 0.820-7.380 μg and 24.600-86.100 μg with the average recovery of 99.3% and 99.5%, respectively. Total saponins and saponins of Tribulus terrestris in Tribulus terrestris from 18 habitats were measured by the methods. Conclusion:The methods are sensitive, accurate and reproducible, and can be used as the quality control methods for Tribulus terrestris. |
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