|
| 气相色谱法测定富马酸替诺福韦二吡呋酯中三乙胺残留量 |
| Determination of Residual Triethylamine in Tenofovir Disoproxil Fumarate by GC |
| 投稿时间：2014-11-23 修订日期：2015-01-04 |
| DOI： |
| 中文关键词: 气相色谱法 三乙胺 富马酸替诺福韦二吡呋酯 |
| 英文关键词:GC Triethylamine Tenofovir disoproxil fumarate |
| 基金项目:国家“重大新药创制”科技重大专项（编号：2009ZX09301-013） |
|
| 摘要点击次数: 976 |
| 全文下载次数: 0 |
| 中文摘要: |
| 摘 要 目的： 建立富马酸替诺福韦二吡呋酯中三乙胺残留的检测方法。方法: 采用气相色谱法，色谱柱为安捷伦DB-624毛细管柱(30 m×0.53 mm,3 μm)，氢火焰离子化检测器(FID)，载气为氮气，流速2.0 ml·min-1，柱温采用程序升温，初始温度为50℃，以10℃·min-1升温至220℃，保持4 min，采用外标法定量。结果:三乙胺浓度在53.02～742.34 μg·mL-1的范围内，峰面积和浓度呈现良好的线性关系，相关系数为0.999 8；回收率为96.52%～102.27%，RSD为2.42%（n=9）。结论: 本法专属性强、重复性好、灵敏度高，可用于富马酸替诺福韦二吡呋酯中三乙胺残留测定。 |
| 英文摘要: |
| ABSTRACT Objective: To establish a method for the determination of residual triethylamine in tenofovir disoproxil fumarate. Methods: The residual treithylamine was determined by GC with an Agilent DB-624 capillary column (30 m×0.53 mm, 3 μm) and an FID detector. The carrier gas was nitrogen and the flow rate was 2.0 ml·min-1. The oven temperature was programming increased. The initial column temperature was 50℃, and then raised to 220℃ at a rate of 10℃·min-1，and maintained 4 min. Triethylamine was quantified by an external standard.Results: The calibration showed a good linearity within the range of 53.02-742.34 μg·mL-1 for triethylamine. The correlation coefficient was 0.999 8. The recoveries were within the range of 96.52%-102.27%. The relative standard deviation (RSD) was2.42% (n=9). Conclusion:The method has good specificity，repeatability and sensitivity, which can be used in determining the content of residual trietbylamine in tenofovir disoproxil fumarate. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
