| ABSTRACT Objective: To investigate the association between the gene polymorphisms of gene ABCC2 and the adverse reactions of mycophenolate mofetil in kidney transplant patients. Methods:Dividing the 236 cases of kidney transplant patients into bone marrow suppression group, gastrointestinal tract reaction group, infection group and control group by adverse reaction type. Using HPLC fluorescence detector to determine the plasma drug concentration of MPA. Using PCR RFLP to determined single nucleotide polymorphisms genotypes of ABCC2 1249G/A, and sending PCR products sequencing directly to verify the accuracy of the agarose gel electrophoresis. Using SPSS 20.0 software to make a statistical analysis in genotype and the patients age, gender, quality, BMI, dialysis duration, cadaveric kidney or living relative kidney and the oral daily doses and concentration of MMF at 3, 6, 12, 24, 36 months. The logistic statistical analysis was used to analyze risk factors for related adverse reactions caused by MMF in post transplant patients. Results:36 cases of bone marrow suppression, 15 cases of gastrointestinal reaction, 26 cases of infection. The mutation frequency of ABCC2 1249G>A was 12.08%. The trough blood concentrations of MMF in GG group were significantly higher than GA group after 12 months postoperative (P＜0.05). The values of BMI were significant difference between GG and GA group (P＜0.01). A Logistic regression model showed that the medication time was the independent risk factor for the bone marrow inhibition.Conclusion:There was no significant association between the ABCC2 1249G>A genotypes and the adverse reactions caused by MMF. |
